Building in silico blocks of gene networks involved in cancer

Lakshya Chauhan, Uday Ram and Kishore Hari’s joint interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Lakshay and Uday are joint-first authors on the recent research paper “Topological signatures in regulatory network enable phenotypic heterogeneity in small cell lung cancer”, published in Elife (2021). In this undergraduate special interview, authors talk about this work, its relevance in the context of the cancer and their motivation for research.

Lakshya is a third-year undergraduate student majoring in Biology at the Indian Institute of Science, Bangalore. His interest lies in classical molecular biology and developmental biology. Lately, he got piqued by interdisciplinary fields like systems biology, biophysics, and biomechanics. Besides work, he can be found playing tennis and multiplayer games.

Uday Ram is a third-year undergraduate student majoring in Physics at the Indian Institute of Science, Bangalore. He is interested in exploring the physics of exotic systems, particularly looking at how collective phenomenon leads to the strange properties of these systems. When not doing physics, his entire focus is on coding in python and playing video games.

Kishore is a third-year PhD student at the Center for Biosystems Science and Engineering, Indian Institute of Science, Bangalore. His interests are using systems biology to understand the diversity in biological systems. Specifically, he is trying to decipher the design principles of regulatory networks that allow cancer cells to bypass various barriers in the body. In his free time, he likes to sing and watch anime.

Listen audio

How would you explain your paper’s key results to the non-scientific community?           

Every single living being is governed by their genetic make-up. This genetic material, DNA or RNA, is further divided into smaller units called genes which encode proteins and other machinery important for functioning. These genes often interact amongst themselves, in large networks. These networks result in all the different cell types in an organism, ranging from neurons (brain cells) to nephrons (kidney cells), from muscles to bones. Going on a tangent, let’s talk about cancer. Cancer cells have been shown to be of multiple types, such as metastatic (the dreaded type that roams around one’s body to find new areas to latch on to), epithelial (the type that latches on and grows) and hybrid types (still under research, but shown to exhibit greater drug resistance and recurrent rates). We focused on subtypes of Small Cell Lung Cancer (SCLC), a deadly cancer with no known cure and abysmal survival rates. In silico methods (simulations done in a mathematical framework) were utilised, and results were compared to the actual patient and experimental data to find anticipated similarities. We also performed a successful reduction in the network size from 33 genes to essentially 4 partners, suggesting that such a large network can be reduced to more intuitive components that still gives one the same subtypes in silico. This is indicative of an idea that a large majority of genes resort to “groups”, where individual groups support/activate amongst themselves but inhibit other groups. Hopefully, this knowledge of such a reduction in large networks is universal in nature and can be applied to other networks/diseases as well. Further, the two major groups acted against each other, and such a “tug of war” could potentially be present throughout biological networks!

Schematic

The metric suggested by us provides a quantifiable measure of the same and could be implemented to find such core principles in a multitude of networks.

What are the possible consequences of these findings for your research area?

If explored further, a universal presence of “groups” could highly impact the time taken for in silico methods for large networks, thus speeding up research. Another possible impact would be an increase in the understanding of roles played by different genes and proteins in various disease stages and types and could further drug/cure development. Such information could potentially also help in finding effective drug targets to subdue various disease subtypes and support faster recovery.

One of the methods used during grouping was the concept of an “Influence matrix”, which suggests that over the course of evolution, gene interactions grow to support the core “principle”, and the entire network evolves around the same, enforcing the principle over aeons. The metric suggested by us provides a quantifiable measure of the same and could be implemented to find such core principles in a multitude of networks.

What was the exciting moment (eureka moment) during your research?

Uday: The most exciting moment I had while doing this project is finding out the fact that this huge network (SCLC network) can be successfully reduced to a 4 node network which preserves all the properties of the bigger network. This implies that big and dense biological networks can in fact be reduced into smaller networks that are both analytically and computationally easy to work with.

Lakshya: For me, it was when I observed that the concept of “Influence matrix” gives the same results as experimental and simulation data. This might not seem huge, but it’s amazing to think that just the network without any simulation and experimental data could give us a clear insight into the properties arising from later. This was quite fascinating for me!!

Kishore: For me, it was when we identified that such a large network has only four states and we figured out that this is due to the nodes of the network forming groups that act together. This implies that the complex interactions are not increasing the complexity of the system, but are working together to preserve the simplicity.

What do you hope to do next?

Uday: At the moment I am exploring different fields of Physics to figure out which one suits me the best. I am certain that I will continue to work on the physics of collective phenomenon.

Lakshya: Currently, I am still exploring various fields of biology. While sure of my resolution to pursue science, I am unsure about which field could be my niche. I hope to stay in more quantitative fields of biology, and over my master’s figure my interests to the best of my capabilities.

As undergraduate students, what motivated you to pursue research?

Uday: Ever since my childhood, I have a desire to face the challenge of solving complex problems. The fact that there are many unsolved questions to be solved made me pursue science. After coming to IISc, I came to know about the existence of exotic systems both in Physics and Biology. These systems are often so complex and diverse yet they follow the same laws of physics. The desire to study them is what motivates me to pursue research.

Lakshya: This would be quite a difficult question to answer. However, if probed, I would say it’s simply curiosity and amazement that drives me. The very nature of science and how evolution drove, from very basic physics-based principles to the rise of sentient creatures and such complex biological systems is something that constantly overwhelms me. I would say it’s this boundless nature of research that keeps my interest piqued.

6.    What was your biggest lesson about interdisciplinary research setup?

Uday: My biggest lesson from this interdisciplinary project is that “to never restrict your thinking to a specific domain of Science while solving a problem”. The questions of “how” and “why” related to a domain-specific question often has a very interdisciplinary answer to it. So as a researcher, it’s always good to go through the work done in other disciplines which will help in the long run.

Lakshya: it is very important to not constraint one’s thought process when it comes to interdisciplinary work, and that’s my biggest takeaway. Constantly reading up other fields, searching for multifaceted components to your research has been an interesting, sometimes exhaustive but definitely fruitful process!

Kishore: Interdisciplinary research thrives on putting various science and engineering disciplines together. It always helps if one has thorough training in one of the disciplines before entering this setup, but communicating with experts of various fields about the problem one is working on becomes a crucial part of interdisciplinary research. Not only is this a quick way to expand the horizons of one’s thought process, but it also makes for a lot of interesting interactions.

7.    Where do you seek scientific inspiration?

Lakshya: YouTube at large is my perfect place for inspiration. Random videos and talks from scientists and educators, and the amazing pace of research and the sheer magnitude of information not known to me is overwhelming, and a constant reminder to keep learning. Documentaries, talks and research showcases help in this!

Uday: Science related articles. Whether in the form of social media posts or news articles, the articles on current research topics is what inspires me to explore more, read more about current research and constantly think about the problems that interest me. Also talking to researchers and attending their talks also inspires me a lot to learn about new things in science.

8.    How do you intend to help Indian science improve?

Lakshya: I think the best way I can help Indian Science improve right now would be to keep honing my skill set and continue on my currently decided path. I do wish to take part in community outreach a bit more, where I would love to teach high school and secondary students about research and science in a more friendly manner than our conventional schools. The way most people are introduced to Biology and science at large is not optimal, often driving students away from even considering research as a career option. This and the opinion that only “smart” people can do science are, in my personal opinion, the two biggest bane to Indian science. Doing my part in changing these would be my way of contributing.

Uday: Coming from a Physics background, I feel that oftentimes students feel that physics is very hard to grasp and is not worth giving a shot. And also because of the huge popularity of JEE and IITs among Senior High School students, a lot of them really don’t get to see the active research happening in the field of Science and how amazing it is. Many coaching institutes just focus on training the students to get marks in exams and won’t make attempts at demonstrating the beauty of Science. So taking part in community outreach programmes and making an effort in bringing awareness about science among parents and students will hopefully make some of the students interested in the field of Science.

Kishore: In my opinion, the best way to improve science in India is by giving undergraduate students a good environment to nurture their research aptitude. The excellent undergraduate programme at IISc is proof of that. Moving forward, I would like to enter academia and prepare teaching modules for undergraduates across the country to expose them to the exciting area of research and improve awareness of various opportunities available in the field.

Reference

Chauhan L, Ram U, Hari K, Jolly MK. Topological signatures in regulatory network enable phenotypic heterogeneity in small cell lung cancer. Elife. 2021 Mar 17;10:e64522. doi: 10.7554/eLife.64522.

Lab website: https://mkjolly15.wixsite.com/cancersystemsbiology

Edited by: Pragya Gupta

Job opening in Biocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited

Hiring for the below position at Biocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited.

Designation: Principal Investigator
Job Location: Bangalore
Department: BBRC-Discovery Biology-Fibrosis
Education: PhD (Must)+ Postdoctoral Experience/industry
Experience: 5 to 8 years

Key position in the Fibrosis Discovery Biology team to enable execution of early and late stage programs in fibrosis.

Technical/functional Skills Required:-

Proficient in immunology assays (primary cell isolation, culture and characterization- whole blood, macrophages, T cells)
Strong background in multicolor flow cytometry, enzymology and cell-based signaling assays
Hands-on experience with translational assays (patient samples) is a plus
Strong understanding of biology and ability to adapt to changing program requirements
Strong biologist with a proven publication record, Strong collaborator
 
If interested or know anyone who would be interested, Please share the resume on gunjan.shah1@syngeneintl.com.
Please mention “BBRC-Fibrosis” in the Subject line.

#syngene #Fibrosis #cellbasedassays #biology

Web-building in the social spiders depends on the availability of prey

Dr. Divya Uma’s interview with Bio Patrika hosting “Vigyaan Patrika”, a series of author interviews. Dr. Divya is a behavioral ecologist at the Azim Premji University (APU), Bangalore. She teaches undergraduate courses in biology. She is interested in concepts like predator-prey interactions, mimicry, sociality in insects and spiders. When she is not teaching, she likes to paint, listen to music and get lost in the forest! Here, she talks about her recent paper titled “Influence of prey availability on web-building in the social spider Stegodyphus sarasinorum (Araneae: Eresidae)” published in the Journal of Arachnology (2021).

Listen audio

How would you explain your paper’s key results to the non-scientific community?

Spiders build their webs to catch prey such as bees, beetles, mosquitoes, moths, grasshoppers etc. It is known that spiders can manipulate the features of their web depending on the type of prey. Spiders can alter the spacing, thickness and stickiness of silk threads of a web. Social spiders can modify their web architecture depending on the amount of prey availability. If there is no prey available, then spiders incorporate a kind of silk known as cribellate silk that enables them to capture prey. If the spiders are full, then they do not invest less in this kind of silk.

Majority of the spiders are solitary—they live by themselves for most part of their lives. But only a few spiders are social. Among ~49,000 species of spiders, only about 25 of them are social. Why do they live in groups?—so that they can collectively hunt larger and numerous prey than their solitary cousins. They can also perhaps gain protection from predators. We study Stegodyphus sarasinorum, a social spider found in India.

Spider web closeup. Photo credits: Maitry Jani

We performed a simple experiment in the lab to examine if S. sarasinorum can alter its web architecture depending on prey availability. Saroja Ellendula, Carol Tresa – two undergraduate students carried out this experiment at the Azim Premji University. We got social spider colonies in Bangalore and gave them frames to build their webs. We then divided these colonies into two groups- the well-fed group that had an abundant supply of grasshoppers daily for a week, and the hungry group which did not get any supply of grasshoppers. We took photographs of their webs and found that the webs of the two groups are very different from each other. The unfed group had a lot more cribellate silk strands than the well-fed group. Unfed spiders put out cribellate silk to catch their prey and these silk strands were very evident. It is intriguing to know how spiders invest themselves in this very energetically expensive process of making the cribellate silk.

Web schematic with spiders

A bunch of spiders modifying their webs may sound trivial—but webs were once thought to be structures that could not be changed once they were built. Several recent studies have shown that webs are influenced by spiders’ own internal state (such as hunger, age of the spider) and external environment (prey availability, presence of predators, wind, rain). Here, we show that the social spiders can fine tune their webs according to the external environment such as prey availability.

What are the possible consequences of these findings for your research area?

We have shown that social spiders can modify their web architecture based on their internal needs. Social spiders are thought to cooperate in web-building, prey capture and taking care of their young ones. Whether individuals in a colony cooperate with each other or do they act as individuals driven by their own internal needs is something that needs to be examined. Our study was done in a controlled set up but we have to examine if this is really true in the field as well.

What was the exciting moment (eureka moment) during your research?

The cribellate silk that these spiders use to build their webs appear as zig zag lines and can be easily identified. We had set up this experiment in the summer in an empty classroom. It was so amazing to be able to accurately classify spider colonies either to the well-fed or the unfed group just by looking at their webs. I had no prior knowledge on the colony and their groups. The stark differences between the two groups were so evident to all three of us. Results are often not so clear cut like this and this really was an exciting moment.

What do you hope to do next?

Social spiders are so fascinating and there are so many aspects to explore about them. We are really interested in examining how spiders in a colony behave, if some are full and some are hungry? Do the spiders that invest more in web-building also catch more prey? Do spiders respond collectively for the benefit of the group or do they behave like individuals? We also want to see if cribellate silk generates different kinds of vibration than non-cribellate silk? How is the vibration carried through the web? I will be delving into these questions further with the help of my collaborators, Dr. Hema Somanathan from the Indian Institute of Science Education and Research Thiruvananthapuram, Dr.Tejas Murthy and Dr. Debraj Gosh from the Indian Institute of Science, Bangalore.

Where do you seek scientific inspiration?

I seek my scientific inspiration from nature, for sure! There are so many fascinating aspects of nature that we do not know. Additionally, interacting with people from different disciplines helps me to think about a topic from a different perspective. I teach at a liberal arts program at APU and here, I often end up chatting with economists, physicists, or historians. These conversations really help me to think out-of -the-box, and incorporate new ideas in our investigations.

How do you intend to help Indian science improve?

Science can be done only once you have a PhD or while you are pursuing a PhD is a false idea. If you are motivated enough at an undergraduate or a high school level, even these students can achieve a lot. Science is expensive and can be done only inside a laboratory is also a false belief. I try to create interest among students about the natural world around them. Once they are hooked, the rest follows! I also strongly believe that communicating scientific discoveries to the general public is important. Engaging common people through social media, podcasts, events where a layman gets to understand and visualize the kind of things scientists do is very crucial and I appreciate the efforts of Bio Patrika for working towards this goal.

Reference

Ellendula, S., Tresa, C., & Uma, D. (2021). Influence of prey availability on web-building in the social spider Stegodyphus sarasinorum (Araneae: Eresidae). The Journal of Arachnology, 49, 141 – 145.

Edited by: Neha Varshney

Job opening in Biocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited

Hiring for the below position at Biocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited.

Designation: Senior Research Associate
Job Location: Bangalore
Department: BBRC-Pharmaceutical Candidate Optimization
Education: M.Pharma
Experience: 0 to 2 years

 Key Responsibilities:

· Prepare in-vitro and in-vivo bioanalytical samples and execute routine bioanalysis tasks in a timely manner with minimal supervision.
· Develop bioanalytical methods and analyze PK/TK/PD samples using LC-MS/MS assays.

Technical/functional Skills: 

· Candidate should have strong understanding of mass spectrometry. Excellent working knowledge of tandem mass spectrometers (eg. Sciex 5500, Waters TQS etc..) would be an added advantage.
·  Good understanding of the drug discovery process and DMPK studies.
·  General knowledge on the different biological sample preparation techniques

If you are interested or know anyone who would be interested, Please share the resume on gunjan.shah1@syngeneintl.com.
Please mention “BBRC-PCO” in the Subject line.

#syngene #bioanalytical

Job opening inBiocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited

Hiring for the below position at Biocon-Bristol-Myers-Squibb R & D Centre, Syngene International Limited.

Designation: Team Leader/Senior Team Leader
Job Location: Bangalore
Department: BBRC-Biopharmaceutics
Education: PhD (Full-Time) (Must) in Pharmaceutics / Industrial Pharmacy / Pharmaceutical Technology or related field(s)
Experience: 6 to 12 Years

To lead a team of scientists for developing preclinical & clinical formulations of new chemical entities (NCEs)..

Key Responsibilities:

1. Provide scientific leadership within Pharmaceutics domain to identify developable drug candidates, which includes:
· Pre-formulation (physicochemical characterization, polymorphism, chemical degradation)
2. Lead projects & guide the team on development of different formulation(s), like solution, suspension, tablet, capsule.
3.     Competency in understanding & assessing biopharmaceutics risks towards potential solubility & absorption issues with NCEs, which includes utilization of various in vitro, in silico & in vivo tools.
4.     Good understanding of various novel drug delivery systems (NDDS) to improve oral bioavailability of a challenging NCE.

If interested or know anyone who would be interested, Please write to me on gunjan.shah1@syngeneintl.com for a detailed Job Description and also share your resume.

Please mention “BBRC-BPH-Team Leader” in the Subject line.

#syngene #Biopharmaceutics

Job opening in Biocon Biologics

#HiringAlert! Here is an opportunity for you to be part of a performance-driven and dynamic team that is on its journey towards transforming healthcare and transforming life.

We at Biocon Biologics are looking for applications from Research and Development (RND) professionals who are passionate and skilled to get it right the first time.

Please visit https://lnkd.in/g-qgybc for applying to RND roles or share your CV with mukesh.guleria@biocon.com before 30th July 2021.

Essential Skills for R&D:

Phys Chem Characterization (Analytical & Characterization)
Cell culture (Upstream)
Drug Substance (Downstream)
Drug Product
Functional Characterization (Bio assay)
Pilot Plant (Downstream & Upstream)

Multiple roles across levels open for applications for our Bangalore & Chennai office.

#BioconBiologics#WeAreHiring#BioPharmajobs#Jobs#Recruitment#BioPharma#CareersAtBioconBiologics#HiringAtBioconBiologics#LifeAtBioconBiologics#Hiring#BioTechnology#HealthCare#Opportunity#JobAlerts

Job opening in immunitoAI

We are #hiring for the position of Scientist – Molecular Biology/Biochemistry !!

Antibodies are an excellent candidate for Targeted Therapy but what prevents them from being rampantly used as a medication today is the time and cost that goes into discovering novel antibodies. At immunitoAI, we want to overcome this hurdle by using Aritificial Intelligence to assist us in Antibody Discovery and Screening.

But it goes without saying that the AI prediction in Biology needs to go hand in hand with inventions in the lab. Biological research and validation with a continuous feedback loop between the dry lab and wet lab lies at the core of our company. We are in the search of talented individuals who can bring in that biological expertise required to complement the computational scientists.

If you are that Someone, motivated to solve real-world problems at exceptional speed and want to take up key positions in the company, becoming a part of the core-team in a fast-growing start-up, follow the link below for a detailed Job Description. Click on the “Apply” button next to the Job Description to fill the application form.

JD and Application Form: https://lnkd.in/gzZQdTx

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SWEATSENSER Dx: A Wearable Device for Tracking Infections from Sweat

Mr. Badrinath Jagannath’s interview with Bio Patrika hosting “Vigyaan Patrika”, a series of author interviews. Badri is a doctoral candidate in the Department of Bioengineering at The University of Texas at Dallas. He completed his Bachelors in Electronics and Instrumentation Engineering in India and obtained Master of Science degree in Biomedical Engineering from Arizona State University in 2015 before joining Dr. Shalini Prasad’s lab at UT Dallas. His research interests include developing wearable and point-of-care devices for precision health. His current research focuses on developing wearable sensors for real-time monitoring of upper respiratory infections and inflammatory bowel disease non-invasively from sweat. Badri currently has 17 peer-reviewed publications among which two of the publications have been recognized as one of the Top 10 articles in Nature Scientific Reports and Inflammatory Bowel Diseases journals. He has received several awards and fellowships for his research work. Badri has given podium presentations at various conferences and has also been an invited Plenary speaker for his work on wearable diagnostics. His research work on infection monitoring wearable sensor has received a lot of recognition and was recently featured at the ACS conference 2021.

Listen audio

How would you explain your paper’s key results to the non-scientific community?

Typically, when a person gets an infection, immune response is triggered releasing certain molecules called ‘cytokines’ as a part of the body’s defense mechanism. Sometimes this defense mechanism leads to excessive or uncontrolled release of multiple cytokines creating a storm-like effect. The impending cytokine storm has rippling effects on the body causing organ damage and ultimately death. Cytokine storms are one of the major causes for complications in COVID-19 and other respiratory infections. There is often a time delay between when cytokine levels get elevated to when the symptoms of infection are experienced. Therefore, it is imperative to capture abnormalities in cytokine levels during onset of infection to prevent any deleterious effects. However, current technologies use blood-based testing methods. They are not suitable for real-time, continuous monitoring, and often take large processing times. These blood tests are typically done only after symptoms appear which can prove fatal due to delay in detection. Therefore, early detection through continuous monitoring can aid in rapid and real-time reporting of any abnormalities in cytokine levels. To overcome current technological shortcomings, a wristwatch like device called ‘SWEATSENSER Dx’, commercialized by EnLiSense LLC, was used to report cytokine levels in real-time from naturally perspired sweat.

The developed SWEATSENSER device comprises of a disposable sensor strip attached to a portable electronic reader. The strip, which contains electrodes, is coated with antibodies to capture specific cytokines. When the device is worn on the wrist, sweat diffuses from the skin onto the sensor strip. Binding of cytokines to their antibodies results in a change in electrical current flowing through the reader. The reader then wirelessly transfers the data to a smartphone app converting electrical signal to an accurate cytokine level. The device can continuously measure the cytokine biomarkers from very low volume (almost 1/10th drop) of naturally expressed sweat. The device is extremely sensitive and can measure four cytokines (tumor necrosis factor-α, interleukin-6, interleukin-8 and interleukin-10) simultaneously. In this pilot study, we were able to differentiate 5 patients with respiratory infections from 10 healthy subjects. These results are significant as this is the first study to report the detection of infection non-invasively from sweat. The findings reported in this work opens new avenues of wearable devices for providing feedback on health status to the user in real-time.

This work was a collaboration between ‘Biomedical Microdevices and Nanotechnology Laboratory’ at The University of Texas at Dallas and EnLiSense LLC. The findings from this work were published in Bioengineering and Translational Medicine.

What are the possible consequences of these findings for your research area?

The ‘SWEATSENSER’ wearable device can be paradigm-shifting in the use of wearable technologies for precision health. The results presented in the study demonstrate a way to track infections non-invasively. Additionally, continuous monitoring of physiological parameters presented in the study can empower users by providing active feedback for self-monitoring. Such wearable systems can be invaluable in providing users with real-time feedback on health status and help physicians make informed decisions on patient treatment strategies.

The results presented in the study demonstrate a way to track infections non-invasively.

What was the exciting moment (eureka moment) during your research?

There are quite a few interesting findings from this study. Current methods use chemical stimulation to induce sweat. However, this causes discomfort and is not a suitable method for quantifying levels of inflammatory molecules. A critical finding was that such external stimulation methods result in under-expression of the actual level of biomarker and do not represent the true level of that in circulation (blood). Further, we were able to quantify the levels of inflammatory cytokines in passive sweat i.e. naturally perspired sweat without any chemical stimulation or exercise. But the “icing on the cake” has been that we were able to detect people with upper respiratory infections through real-time monitoring of biomarkers in sweat. This is the first-of-a-kind demonstration of technological viability to detect infections non-invasively. Such a result shows a lot of promise and provides hope for rapid and early detection during pandemics and epidemics.

What do you hope to do next?

We are collaborating with hospitals to validate the clinical performance of the device. We would like to further expand our studies by testing on large patient population with various upper respiratory infections.

Where do you seek scientific inspiration?

Inspiration to me comes from learning and observing the world around me. I get motivated while reading some innovative research articles and discussions with my peers helps me think out of the box. When I come across certain unique experiments from research papers, it gives me a lot of enthusiasm . But importantly, my failures motivate me the most. They keep me determined, humbled and perseverant in pushing my limits to achieve my goals.

How do you intend to help Indian science improve?

One of the holistic ways for progress in science is by collaboration. Industry collaborations help in translation of research from an academic lab to the market for which this work is an excellent example. I believe Indian science would benefit greatly with such academic-industry partnerships. I would like to contribute in whatever capacity I can by exchange of information with academic researchers in India on my experience of collaboration with industry for translation of research from an academic setting.

Reference

Badrinath Jagannath, Kai-Chun Lin, Madhavi Pali, Devangsingh Sankhala, Sriram Muthukumar, Shalini Prasad. Temporal profiling of cytokines in passively expressed sweat for detection of infection using wearable device. Bioengineering & Translational Medicine (2021): e10220. https://doi.org/10.1002/btm2.10220

Corresponding authors: Dr. Shalini Prasad, The University of Texas at Dallas; Dr. Sriram Muthukumar, EnLiSense LLC.

Website: https://www.utdbmnl.com/

Edited by: Dolly Singh

Job opening in Laurus Bio

Hiring!! For Laurus Bio – Quality control.
Openings for –
0-1 years – internship.
2- 5 years – Experienced.

Qualification: M.Sc. Biotechnology/ Biochemistry/ Lifescience/ Biological Science.

Experience on HPLC, UV – Enzyme assays, Raw material testing, packing material testing , Water analysis, QMS investigation, Instrument Calibration.

Location : Bangalore / Tumkur.

Share resume : shivakumar.kt@laurus.bio

Job opening at Biocon-Bristol-Myers-Squibb R & Centre, Syngene International Limited

Hiring for the below position at Biocon-Bristol-Myers-Squibb R & Centre, Syngene International Limited.

Designation: Associate Scientist/Research Investigator
Job Location: Bangalore
Department: Discovery Biology, BBRC
Educational Qualification: Fresh PhD or MSc in Biotechnology/Life Science background
Experience: 3-4 years relevant experience 

Key Responsibilities:

Performing Biacore experiments; crystallization and solving crystal structures; maintenance of linux boxes and softwares; Scripting support as and when needed

Technical/functional Skills:
Hands-on experience with one or more of the following –
o SPR,
o Octet,
o ITC,
o protein crystallization
o Knowledge of working with Linux system

If interested or know anyone who would be interested, Please share the resume on gunjan.shah1@syngeneintl.com
Please mention “BBRC-Protein Science” in the subject line while sharing the resume.

#jobopportunity #Syngene #Bangalore #Proteinscience #proteincrystallization #biophysicaltechniques