Job opening in Meticulous Research

Meticulous Research is hiring for a Research Associate – Healthcare position.

Qualification: MBA, B.Pharma, M.Pharma

Skills required: Good communication

Years of experience: 0-1 year

Location: Wakad, Pune

Immediate joiner will be preferred.

If you are interested, please share your updated resume to bhagyashree.t@meticulousresearch.com.

Job opening in Affigenix – Chennai

Affigenix Biosolutions Private Limited is hiring.

Qualification: Undergraduate and Master’s degree from Biochemistry, Microbiology and Biotechnology field.

Responsibility: Development of novel diagnostic kits.

Location: Diagnostic kit manufacturing facility located at Alathur near Chennai, Tamil Nadu.

Check Affigenix website for more information: www.affigenix.com.

If interested, please share your updated CV with cover letter to info@affigenix.com.

Bio Patrika interviews Dr. Sengupta on her thoughts about “directionality of proteolysis”

Dr. Bhaswati Sengupta’s interview with Bio Patrika hosting “Vigyan Patrika“, a series of author interviews. Dr. Sengupta is currently working on Nucleosome dynamics and chromatin remodelling using smFRET as a postdoc in the Department of Chemistry at Pennsylvania State University, USA. She did her PhD from the lab of Prof. Pratik Sen, Department of Chemistry, Indian Institute of Technology Kanpur, India. She published last part of her PhD work titled “Fluorescence correlation spectroscopy as a tool to investigate the directionality of proteolysis” as the first author in International Journal of Biological Macromolecules (2020).

How would you explain your paper’s key results to the non-scientific community?

Proteins are a long chain of amino acid molecules connected by a peptide bond. Proteolysis is a significant part of the digestion system where the protein gets cleaved into smaller peptides. In this paper, we have used human serum albumin (HSA) as a model protein and papain, chymotrypsin, and trypsin as enzymes to digest HSA. Being a long-chain protein, HSA can be cleaved from either side. In this work, we have used fluorescence correlation spectroscopy (FCS) to understand exactly from which side these enzymes cleave HSA. FCS enables us to measure the change in the size of the fluorescently labelled fragments. As the digestion proceeds, the size becomes smaller, which we measure by FCS. By preferentially labelling to two sides of the protein chain with fluorescent dyes, we can see from which side the digestion process starts. The principle of the measurement is shown in Scheme 1.

Scheme 1: Monitoring protein digestion by single molecule technique.

HSA is a large protein having three domains. For HSA, we found out that digestion starts from the domain I for all three enzymes under consideration. Fluorophore 1 (TMR) slows the faster decrease of size as compared to fluorophore 2 (NPCE) labelled one, which indicates that the fragment size decreases faster for the domain I indicating that digestion starts from the domain I. We have also performed reverse-phase high-performance liquid chromatography (RP-HPLC) to cross-check the findings, supporting our results.

What are the possible consequences of these findings for your research area?

Several techniques enable the measurement of digested fragments after proteolysis like mass spectrometry, HPLC, etc. The uniqueness of the proposed approach is its ability to tell directionality using a minimum amount of protein. FCS is sensitive and can be successfully used for picomolar to nanomolar sample concentrations. This is a new application of the single-molecule research that can be used to understand proteins’ digestion profile.

“[…] I see you are now able to think of novel ideas and design the experiments; your training has been completed”

What was the exciting moment (eureka moment) during your research?

There is more than one Eureka moment associated with this specific work. This was the last project I worked on during my PhD. When I conceived the idea and shared with my boss, he said, “I see you are now able to think of novel ideas and design the experiments; your training has been completed”. That was some words of motivation before I could start the next venture. When I started working, I was unsure if I would see any difference in the digestion profile monitored by two different fluorophores labelled at two other locations. If there was no difference, there was no specific conclusion, and it wouldn’t have been as fascinating as the current finding. So when I observed a difference in the digestion profile, I was pretty excited, which was definitely the second eureka moment. The third eureka moment would be when the reverse phase HPLC supported the single-molecule measurements. That was the moment when we found proof that whatever we see in single-molecule measurement is valid.

What do you hope to do next?

Regarding the piece of work, I talked about, I am not following up on that anymore. We had several ideas of trying the technique on different proteins and used different enzymes and different environments. Also, to use drugs to see if they affect the digestion profile etc. But unfortunately, I had to leave to join a postdoc and couldn’t continue with it. If someday I get a chance, I would like to work on the extended ideas. 

Where do you seek scientific inspiration?

The best (or the worst) thing being a researcher is that I don’t know what waits ahead, and from my early childhood, I have felt tremendous attraction towards what is unknown. It feels exciting to delve into the strange world, which is why I keep loving science. Besides, I am blessed to have supervisors (PhD and postdoc) who motivate and have always believed in my ability and dedication. The excitement of discovering something new is often inexplicable. All these factors keep me going, and I wish to be roaming around the field of science forever.

How do you intend to help Indian science improve?

Honestly, “improving Indian science” is a huge goal, and I don’t think I have reached the stage to think about it. Right now, I am doing postdoctoral research and wish to set up my lab in India someday. Provided I can do that; my next goal would be to design experiments with minimum budget and resources possible given the poor condition of the academic budget in India. The next target would be to equip the lab with efficient and budget-friendly instruments. Once all these are in place, one can use single-molecule experiments for several biological and chemical applications using a minimum sample amount. This will cut down the cost of buying or producing expensive samples again and again.

Reference

Sengupta B, Das N, Singh V, Thakur AK, Sen P. Fluorescence correlation spectroscopy as a tool to investigate the directionality of proteolysis. Int. J. Biol. Macromol. 2020; 164: 2524-2534.

Email: bhaswati1487@gmail.com

Job opening in Biocon Biologics

BIOCON Biologics is Hiring.

Department: Biosimilars – MSAT (Downstream)

Experience: 7-12 years

Qualification: MSc/MTech/PhD

Responsibilities:

  1. To manage MSAT Lab functions on routine basis for Downstream related activities.
  2. To ensure product life cycle management.
  3. To handle continuous verification of process, deviation and out of specifications.
  4. To evaluate the alternate vendors for critical raw materials and consumables as required.
  5. Contribute at team discussions of CMC and other decision-making forums for development, clinical and commercial manufacturing.
  6. Review Technology transfer of manufacturing process to CMOs (outside parent site).
  7. Scientific knowledge on microbial downstream processes.

Interested candidates with relevant experience may share profile at sushmitha.101@biocon.com.

Job opening in Kemwell Biopharma

Kemwell Biopharma is hiring experienced professional for Bangalore location.

Position: Executive, Quality control (QC)

Skills required: Experience in in-vitro Bioassays and potency determining ELISA

Experience: 1-3 years

Requirement: Immediate

If you are interested, please share your updated resume to debatree.chakrabarty@kemwellpharma.com

Job openings in CuraTeQ Biologics

Six job openings in CuraTeQ Biologics – Hyderabad.

Skill required: Cell culture, purification and analytical sciences functions.

Qualification: PhD in relevant study area, pursued post-doctoral research for a period of at least 2 years and/or 2-3 years of post- PhD industry work. Overseas PhDs from top 30 Universities (QS Ranking) can apply without post- doctoral experience.

If you are interested, please share your updated resume to HR Nobby Paul (nobby.paul@curateqbio.com).

Job openings in Biocon Biologics – Chennai

Multiple job openings in R&D department, Biocon Biologics – Chennai.

Skill required: Cell Culture, MAb’s, well versed with R&D upstream process, Mammalian cell culture techniques, Relevant experience in R& D process.

Working experience in Biologics/Biosimilar/Biopharma industry.

Years of experience: 8 to 12 years

Qualification: Ph.D/Master’s in Biotechnology

Location: Chennai

If you are interested, please share your updated resume to shilpa.BBHRDCON11@biocon.com

Job opening in Aurigene

Department: Cells & Molecular Biology

Work Location: Bangalore

No. of position: 2

Qualification: M.Sc. with 3-5years’ experience

Key responsibilities:  

  • To work as part of drug discovery project teams in both internals and collaboration programs.
  • Conduct cell based assays and molecular biology experiments using primary cells and cell lines.
  • Establish various high throughput in-vitro assays for screening of molecules such as proliferation assay, ELISA, Western Blot analysis, In-cell-western assay, reporter assay, qPCR etc.
  • Experience with target validation experiments using techniques such as knock down using siRNA, shRNA & CRISPR is an added advantage.
  • Able to troubleshoot experiments, analyse data and prepare scientific presentations.
  • Independently design and execute experiments and present data to cross functional teams.

Interested candidates with relevant experience may send their resume to preeti_5@aurigene.com.

For more details, please call at +91-9590737048.

Job opening in docplexus

Job opening for Senior medical writer in docplexus

Qualification: MBBS, BHMS, BAMS, PhD and Post-graduate degree in lifesciences discipline

Experience: 3+ years

Requirements:

  • Excellent writing, editing and organizational skills.
  • An excellent understanding of the pharmaceuticals industry and drugs. development process.
  • Ability to manage multiple writing projects at one time.
  • Ability to work independently as well as in a team environment.

Interested candidates with relevant experience may send their resume to neha.shingote@docplexus.in.