Restoring the Wnt/β-catenin signaling rescues bone defects in Job syndrome

Dr. Prem Swaroop Yadav was born in the Chaubeypur village of Varanasi district of Uttar Pradesh, India. He finished his schooling from the Govt. Queens Inter College (GIC), Varanasi. After completing his Bachelor’s and Master’s degrees from the Udai Pratap Autonomous College in Varanasi, he joined the PhD program at the Biological Sciences and Bioengineering (BSBE) Department, IIT Kanpur, India in 2016. He pursued his PhD under the supervision of Prof. Amitabha Bandyopadhyay. For his post-doctoral research, he moved to Boston, USA and joined the laboratory of Prof. Yingzi Yang at the Harvard School of Dental Medicine (HSDM) where he studied the pathophysiology of skeleton-related genetic disorders. Recently, he has joined Prof. Marie B. Demay’s group as a Research Fellow in Medicine at the Massachusetts General Hospital (MGH) & Harvard Medical School (HMS), Boston, USA. In Demay’s lab, he is investigating the role of key signaling pathways involved in hypertrophic differentiation of chondrocytes (which are the cartilage forming cells) and their endocrine regulation during bone development.

Scientific Zeal

Dr. Swati Priya is an ICMR Postdoctoral Researcher from National Institute of Immunology. She graduated from Miranda House (Delhi University) in Zoology(H) and post-graduated in Biomedical Science from Dr. B. R. Ambedkar Centre for Biomedical Research (Delhi University). She started her research career in 2012 when she joined for her Ph.D research work at National Institute of Immunology. She has worked extensively in the field of miRNA and Colon Cancer. She worked on the collaborative projects, where she deciphered the role of Histone activation marks in the presence and absence of Bloom Helicase and its global role in gene expression. She has also worked on project involving the role of BLM in c-Jun degradation with the help of FBW7 alpha. Her extensive work also helped in contribution towards recruitment studies of BLM on DNA double stranded break sites. Her current research work involves deciphering the DNA damage sensitive miRs upregulation in rectal cancer and their regulation and downstream target genes. Her work further elaborates and gives insight into BLM involvement in the expression of miRs via resolving G4 quadraplex present on miRS TSS in B cell lymhoma.

Argonaute navigating the balance between protein translation and small RNA synthesis

First author interview: Dr. Meetali Singh is currently working as a Pasteur-Roux-Cantarini Postdoctoral fellow in the Department of Developmental and Stem Cell Biology, Institut Pasteur, Paris with Dr. Germano Cecere. She obtained her B.Sc. honors degree in Biomedical Sciences from Delhi University and then joined the Indian Institute of Science, Bangalore for the Integrated PhD program where she did her PhD in Prof. Utpal Tatu’s lab at the Department of Biochemistry. During her PhD, Dr. Singh studied the role of the chaperone Hsp90 in the life cycle and virulence of protozoan parasites and investigated the evolution of the Hsp90 complex and its function in primitive protozoa, primarily using parasites like Entamoeba, Trichomonas and Theileria as models. For her post-doc research, Meetali transitioned to explore sRNA-mediated epigenetic gene regulation and inheritance in the free-living worm, C. elegans, in the lab of Dr. Germano Cecere at Institut Pasteur, Paris. She is currently investigating the biogenesis and function of endo-siRNAs and their transmission from soma to germline by integrating proteomic, biochemical, and high-throughput genomic approaches. In her free time, she loves to read, travel and very recently picked up abstract acrylic painting as a hobby.

OmpV- A step towards vaccination against salmonellosis

First author interview: Dr. Deepinder Kaur was born and brought up in Patiala, Punjab. She did her bachelor’s in biotechnology from Khalsa College, Patiala, followed by masters in biotechnology from Thapar University, Patiala. She has completed her Ph.D. under the supervision of Dr. Arunika Mukhopadhaya, an Associate Professor in the Department of Biological Sciences, IISER Mohali. The title of her Ph.D. thesis is “Understanding the role of OmpV, an outer membrane protein of Salmonella Typhimurium towards bacterial pathogenesis and host immune activation.” Here she talks about her work “Salmonella Typhimurium adhesin OmpV activates host immunity to confer protection against systemic and gastrointestinal infection in mice” recently published in Infection and Immunity.

Building a subcellular clamp on the plasma membrane

Dr. Tushna Kapoor’s interview with Bio Patrika hosting “Vigyaan Patrika,” a series of author interviews. Tushna did her bachelor’s in Biochemistry, from Sri Venkateswara College, University of Delhi. Subsequently, she joined the Tata Institute of Fundamental Research (TIFR), in Mumbai, where she worked in the laboratory of Prof. Krishanu Ray, as an integrated Ph.D. student. Tushna’s doctoral work involves deciphering the role of the cell membrane, cytoskeleton, and cell adhesion remodeling during spermatid maturation in Drosophila. Her recent paper shows that the somatic cell plasma membrane folded around maturing spermatid heads can recruit a repertoire of proteins that assemble a membrane-cytoskeleton substructure, which clamps together the spermatid heads, and also prevents them from piercing through the somatic cell membrane. This work highlights novel roles of membrane-actin interactions, in an in vivo tissue system. Tushna is currently working at the Institute of Science and Technology (IST), Austria. Here, Tushna talks about her work ‘An actomyosin clamp assembled by the Amphiphysin-Rho1-Dia/DAAM-Rok pathway reinforces somatic cell membrane folded around spermatid heads’ published in Cell Reports

Prion-like p53 amyloids and their link to cancer pathogenesis

Dr. Ambuja Navalkar’s interview with Bio Patrika hosting “Vigyaan Patrika,” a series of author interviews. Dr. Ambuja is currently working as an Institute postdoctoral fellow at IIT Bombay. She has completed her doctoral studies under the guidance of Prof. Samir K. Maji in the department of Biosciences and Bioengineering, Indian Institute of Technology Bombay. Previously, she has pursued her M.Sc degree in Biotechnology from the Maharaja Sayajirao University of Vadodara, Baroda, and her B.Sc degree in Biotechnology from Ruia College, University of Mumbai. She is interested in exploring the protein aggregation linked with cancer and neurodegenerative disorders. Apart from research, she enjoys reading, good food, and solving puzzles. She wants to pursue scientific research focused on fundamental processes in cells which can answer disease-relevant questions. Here, Ambuja talks about her work on prion-like p53 amyloid formation and its link to cellular transformation leading to tumorigenesis published in the Journal of Cell Science.

Gut pathogens deploy anti-apoptotic pathway to maintain infectious foothold in their host

Gad Frankel. She obtained her M.Sc. degree from Department of Molecular Biology and Biotechnology, Tezpur University, Assam. She did her Ph.D. under the supervision of Prof. Utpal Tatu, Department of Biochemistry, Indian Institute of Science, Bangalore. During her Ph.D., she reported the misdiagnosis of a multidrug resistant fungal pathogen Candida auris. She did the first draft genome of the pathogen and also developed a PCR based diagnostic method to accurately identify it. Further she also worked extensively on heat shock proteins in fungal pathogens such as Candida albicans and Cryptococcus neoformans. She published her research findings in three articles: “Draft genome of a commonly misdiagnosed multidrug resistant pathogen Candida auris” in BMC genomics (2015), “Heat shock protein 90 localizes to the surface and augments virulence factors of Cryptococcus neoformans” in Plos Neglected Tropical Diseases (2017) and “Structural basis for species-selective targeting of Hsp90 in a pathogenic fungus” in Nature Communications (2019). Her recent work on a bacterial effector protein of enterohaemorrhagic Escherichia coli and related pathogens was published in Cellular Microbiology.

A Talk on Nanotheranostic System for Targeted Breast Cancer Therapy developed by IISER-K

Dr. Tapan Kumar Dash and Diptendu Patra’s joint interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Tapan and Diptendu are joint-first authors on the recent research paper “Hetero-Trifunctional Malonate-Based Nanotheranostic System for Targeted Breast Cancer Therapy” published in ACS Appl Bio Mater. In this interview, they talk about this work done with Prof. Raja Shunmugam at Indian Institute of Science Education and Research Kolkata.

Mechanobiology: Mechanical force-induced selective cancer cell killing

Dr. Ajay Tijore’s interview with Bio Patrika hosting “Vigyaan Patrika”, a series of author interviews. Dr. Tijore completed his doctoral studies in stem cell bioengineering field from Nanyang Technological University, Singapore. He is currently working as a Post-Doctoral Research Fellow in the lab of Prof. Michael Sheetz at Mechanobiology Institute, National University of Singapore. His research focus lies in cancer mechanobiology area. Currently, he is investigating the effect of physiologically relevant mechanical forces on cancer cell growth using microfluidic and ultrasound devices. Interestingly, he found that mechanical stretching promotes cancer cell killing without damaging normal cells. To enable clinical mechanical therapy, Dr. Tijore, Prof. Sheetz and colleagues have developed a non-invasive ultrasound-based technology, in which ultrasound generated mechanical forces induce cancer cell killing. Based on the encouraging results, they have recently established the company Mechanobiologics, Inc to systematically develop the ultrasound technology for cancer treatment. Here, Dr. Tijore talks about his findings that has been recently published as a research article entitled “Selective killing of transformed cells by mechanical stretch” in the journal ‘Biomaterials’ in 2021.