Impact of mAb Aggregation on its Biological Activity

Dr. Rozaleen Dash’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Dash completed her PhD in Biochemistry from All India Institute of Medical Sciences, New Delhi then, she joined as Scientist- B at Maulana Azad Medical College, New Delhi. Currently, she is doing Post-Doc as a ICMR-RA fellow in the Professor Anurag S Rathore Lab, Department of Chemical Engineering, Indian Institute of Technology Delhi, India. She has expertise in Functional Characterization of biotherapeutics, Comparability of Biosimilars and Drug immunogenicity. Here, she talks about her co-first author research article titled “Impact of mAb Aggregation on Its Biological Activity: Rituximab as a Case Study” published in the Journal of Pharmaceutical Sciences (2020).

Women in science: I am a woman, a wife, a mother, and a scientist!

Dr. Manveen K Sethi serves as Instructor of Biochemistry at the Boston University School of Medicine, USA. She did her Ph.D. at Macquarie University (MQ), Australia, under the primary supervision of Dr. Morten Thaysen-Andersen,where she utilized mass spectrometry proteomics and glycomics analysis to understand underlying molecular mechanisms in colorectal cancer . After her Ph.D., she joined Boston University School of Medicine (BUSM), USA, as a postdoctoral associate under Prof. Joseph Zaia, where she is currently employed in a research faculty-track position of Instructor. Her research work involves identifying and characterizing biomolecules such as proteins and glycans using mass spectrometry techniques and utilizing this information to understand biomolecular deregulation in human diseases, such as cancer and Alzheimer’s disease. Recently, she received a Bright Focus Foundation fellowship award to investigate extracellular matrix changes in Alzheimer’s disease.

Bio Patrika interviews Dr. Sethi on her thoughts about “Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis”

Dr. Manveen K Sethi’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Sethi serves as Instructor of Biochemistry at the Boston University School of Medicine, USA. She did her Ph.D. at Macquarie University (MQ), Australia, under the primary supervision of Dr. Morten Thaysen-Andersen, an internationally recognized researcher in Glycobiology. During her Ph.D., she forayed into colorectal cancer (CRC) research. After her Ph.D., she was offered a postdoctoral research fellowship at Boston University School of Medicine (BUSM), USA, under Prof. Joseph Zaia, an internationally-acclaimed leader in mass spectrometry (MS) and Glycoscience, where she is currently employed in a research faculty-track position of Instructor. Her research work involves identifying and characterizing biomolecules such as proteins and glycans using mass spectrometry techniques and utilizing this information to understand biomolecular deregulation in human diseases, such as cancer and Alzheimer’s disease. Here, Manveen talks about her work titled “Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysis” published as a first author in Molecular Omics (2020).

Bio Patrika interviews Dr. Joshi on her thoughts about “Orthogonal tools for analytical biosimilarity assessment of biotherapeutics”

Dr. Srishti Joshi’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Joshi completed her PhD in Molecular Biology and Biochemistry from Massey University, NZ in 2015. After a brief Post Doc experience at the Systems Biology lab at Korea University, she joined DBT-COE-CBT (IIT-Delhi) in 2017 an Institute Post Doctoral Fellow in the lab of Prof. Anurag Rathore at the Centre of Excellence, Biopharmaceutical Technology (DBT-COE-CBT), Indian Institute of Technology, Delhi. She has been learning and exploring the domain of Analytics in Biotherapeutics, since. She published papers titled “An application of Nano Differential Scanning Fluorimetry for Higher Order Structure assessment between mAb originator and biosimilars: Trastuzumab and Rituximab as case studies” and “Monitoring size and oligomeric-state distribution of therapeutic mAbs by NMR and DLS: Trastuzumab as a case study” as first author in Journal of Pharmaceutical and Biomedical Analysis (2020).

Bio Patrika Volume 2, Issue 1

First issue of Bio Patrika 2021 is available now as combined PDF with compilation of 14 author interviews, 3 Healthcare startup founder interviews and 1 article on awards published online between December 2020 to January 2021.

Bio Patrika interviews Dr. Mishra on her thoughts about “how dengue virus activates human microglial cells?”

Dr. Ritu Mishra’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Mishra has completed her Ph.D from CCMB, Hyderabad and moved to Max Planck Institute of Molecular Biomedicine, Germany for her post-doctoral work. She is currently working as DST-INSPIRE faculty at National Institute of Immunology, New Delhi. She has expertise in Virology, immunology and neurological research and have done extensive research on HIV, Dengue, JEV and SARS-CoV-2 viruses. She published paper titled “Dengue Virus Degrades USP33–ATF3 Axis via Extracellular Vesicles to Activate Human Microglial Cells” as a first author in The Journal of Immunology (2020).

Bio Patrika interviews Dr. Jaswal on her thoughts about “role of long-chain fatty acids metabolism in stress response”

Dr. Kanchan Jaswal’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Jaswal has done Ph.D. with Dr. Rachna Chaba at the Indian Institute of Science Education and Research (IISER) Mohali. In her PhD, she investigated the interconnection between carbon metabolism, electron transport chain and envelope redox homeostasis in Escherichia coli. In future she would like to address key questions in the area of host-gut microbiome interactions. As part of her PhD, she published a paper entitled “Metabolism of long-chain fatty acids affects disulfide bond formation in Escherichia coli and activates envelope stress response pathways as a combat strategy” as a first author in PLOS Genetics (2020).

Bio Patrika interviews Dr. Trivedi on her thoughts about “CRISPR-Cas9 based genome engineering resource for studying lipid signalling”

Dr. Deepti Trivedi’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Trivedi heads the Fly Facility at Bangalore Life Science Cluster, Bangalore. Deepti’s paper as a first author in eLife journal (2020) entitled “A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signalling” is a collaboration between Fly Facility and Prof Raghu Padinjat’s laboratory at National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore.

Bio Patrika interviews Dr. Chakrabarti on her thoughts about “injury mediated training of the innate immune system”

Dr. Sveta Chakrabarti’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Chakrabarti is currently working as an India Alliance (Wellcome/DBT) Postdoctoral Fellow in Prof. Sandhya S. Visweswariah at the Department of Molecular Reproduction, Development, and Genetics, Indian Institute of Science, Bangalore. Dr. Chakrabarti published a paper entitled “Intramacrophage ROS Primes the Innate Immune System via JAK/STAT and Toll Activation” as a first and senior author in Cell Reports journal (2020).

Bio Patrika interviews Dr. Datta on her thoughts about “method to rapidly identify target sites of HIV virus neutralizing antibodies”

Dr. Rohini Datta’s interview with Bio Patrika hosting “Vigyan Patrika”, a series of author interviews. Dr. Datta is currently a Postdoctoral Research Fellow in the lab of Jin Billy Li at Department of Genetics, Stanford School of Medicine, Stanford University, USA. Work presented here is done as part of her PhD thesis in the lab of Prof. Raghavan Varadarajan at Molecular Biophysics Unit, Indian Institute of Science, Bangalore. Dr. Rohini published a paper entitled “A facile method of mapping HIV-1 neutralizing epitopes using chemically masked cysteines and deep sequencing” as a first author in Proc Natl Acad Sci USA journal (2020).